Transcutaneous organ function measurement

ABSTRACT

A sensor plaster ( 116 ) for the transcutaneous measurement of an organ function, more particularly of a kidney function, is proposed. The sensor plaster ( 116 ) comprises at least one flexible carrier element ( 134 ) having at least one adhesive surface ( 138 ) which can be stuck onto a body surface. Furthermore, the sensor plaster ( 116 ) comprises at least one radiation source, more particularly a light source ( 142 ), wherein the radiation source is designed to irradiate the body surface with at least one interrogation light ( 162 ). Furthermore, the sensor plaster ( 116 ) comprises at least one detector ( 146 ) designed to detect at least one response light ( 176 ) incident from the direction of the body surface.

The invention relates to sensor plasters, sensor systems, kits, and theuses thereof, and a method for producing a sensor plaster, a method forthe transcutaneous measurement of an organ function and a use of afluorescence-marked indicator substance for producing a diagnostic aid.Such devices and methods can be used more particularly for measuring akidney function, more particularly for measuring a glomerular filtrationrate. However, other applications are also conceivable, in principle.

In the clinical and preclinical field, determining various organfunctions is accorded great importance since, for example, correspondingtherapies or medications can be controlled in accordance with said organfunctions. The invention is described hereinafter substantially withregard to the kidney function. In principle, however, other applicationsare also conceivable in which the function of a particular organ can bedetected by means of determining a temporal profile of an indicatorsubstance.

In kidney diagnostics, the quantitative and qualitative functionaltesting of the kidneys is of great significance. One indicator of thekidney function is the so-called glomerular filtration rate (GFR). Thisshould be understood to mean indirectly the amount of primary urineproduced by the glomeruli of the kidneys per unit time.

For quantifying the glomerular filtration rate, several methods areknown from the prior art and medical practice. One class of methods,into which the present invention is also to be classified, is based onthe use of one or a plurality of indicator substances. Thus, inprinciple, it is possible to use any desired exogenous or endogenoussubstances in the blood as indicator substances which are at leastpredominantly removed from the blood on account of the kidney function.This means that the indicator substance is removed from the body atleast predominantly by the filtration effect of the glomeruli, in whichcase substantially neither tubular secretion nor resorption from theprimary urine takes place. The removal of the indicator substance fromthe blood is also referred to as renal clearance. In this case,clearance is generally designated as that amount of plasma inmilliliters which is freed of the indicator substance by the kidneys perminute.

Various exogenous and/or endogenous indicator substances are known fordetermining the renal clearance and hence the glomerular filtrationrate. Examples of endogenous indicator substances are creatinine orcystatin C. Various exogenous indicator substances are also known fromthe prior art. More particularly, saccharides, e.g. polyfructosans, canbe used as indicator substances. Examples of suitable indicatorsubstances are disclosed in WO2001/85799 or WO2006/32441. It isgenerally possible to have recourse to this prior art in the context ofthe present invention as well.

From a metrological standpoint, one of the challenges consists, inparticular, in determining the concentration profile of the indicatorsubstance and thus the clearance thereof. Numerous different methods bymeans of which the clearance can be detected metrologically are compiledin WO 99/31183. Thus, some of the methods are based on the fact thatblood and/or urine samples are taken at regular or irregular intervals,and the concentration of the marker substance is determinedanalytically, for example by means of enzymatic detection methods. Othermethods are based on the use of radioactive indicator substances and/orX-ray contrast media. The acceptance of such indicator substances by thepatient is generally low, however. Methods based on determining therenal clearance by means of chemical or biochemical analysis or on theuse of radioactive indicator substances are generally complex andburdened with high errors. In routine clinical practice, therefore, inmany cases the kidney function is estimated on the basis ofapproximation formulae, which, however, are likewise very inaccurate andcan have error tolerances in the range of 30 to 40%.

The prior art therefore likewise discloses methods based on the use offluorescent markers. In this case, use is made of indicator substancesmarked with dyes that can be detected optically. By way of example,these can be fluorescent markers which are admixed with the indicatorsubstances or bonded to the indicator substances, for example bycovalent bonding. Examples of marked indicator substances are describedin WO2001/85799 or WO2006/32441, in which case it is possible to haverecourse to these marked indicator substances, for example, in thecontext of the present invention.

In the latter methods mentioned, therefore, an optical signal is used asa measure of the concentration of the indicator substance. In this case,the respective concentration of the indicator substance can be deducedfor example from a known relation between the optical signal and theconcentration. Said known relation can be, for example, of an empirical,semi-empirical or analytical nature, for example a relation determinedby means of calibration measurements. Thus, in DE 100 23 051 A1, forexample, the indicator substance used is sinistrin marked withfluoresceinisothiocyanate (FITC). In this case, a noninvasive,transcutaneous measurement of the FITC fluorescence signal by means of anoninvasive measuring head is described, inter alia. Said measuring headis configured as a fiber-optic measuring head in which an external lightsource, via an optical fiber, illuminates the skin and excites theFITC-sinistrin molecules contained therein. The fluorescent lightemitted by the FITC is in turn picked up by means of optical fibers andforwarded to an external detector.

However, the measurement of the fluorescence signals as described in DE100 23 051 A1 is extremely complex in terms of apparatus technology.This is because it is necessary to provide complex spectrographs inorder to evaluate the measurement signals. Moreover, a fiber-opticsystem is required which, on account of the associated losses ofexcitation light, necessitates the use of highly intensive lightsources, more particularly lasers. The fiber-optic system, together withthe complex light sources and lasers, has the effect, however, that ameasurement of the renal clearance cannot be carried out in an ambulantmanner or by means of portable equipment, but rather practicallyexclusively in optical laboratories specifically designed for thispurpose.

Numerous further analysis systems which, in principle, are also suitablefor portable equipment are generally known from other fields of medicaldiagnostics. Thus, US 2004/0210280 A1, for example, describes aplaster-like system which can be used for transdermal therapy anddiagnosis. Said document proposes, inter alia, that the systemindependently collects and takes up fluid samples from the skin.

In A. Pais et al.: High-sensitivity, disposable lab-on-a-chip withthin-film organic electronics for fluorescence detection, Lab Chip,2008, 8, 794-800, a disposable lab-on-a-chip test element is proposed.The latter is based on an organic light-emitting diode and an organicphotodetector. The test element is configured as a microfluidic testelement and is able to analyze liquid samples by means of fluorescencedetection.

DE 10 2004 048 864 A1 describes an analytical test element with wirelessdata transmission which is used for determining the concentration of ananalyte from a body fluid. Said document proposes configuring at least aportion of the electrical components of the system on the basis ofpolymer electronics.

US 2006/020216 A1 describes a portable health management apparatus thatcan be used, in particular, for a blood pressure measurement. Saiddocument proposes, inter alia, measuring the movement of the bloodwithin a blood vessel by means of light absorption of light incidenttransdermally.

Methods and devices in which a skin surface is irradiated with lightfrom a light source are likewise known from the field of medicaltherapeutics. Thus, a device for the photodynamic therapy of skin cancerdiseases is described, for example, in I. Samuel: “Light fantastic”,Materials World, Aug. 2007, 28-30. This prior art proposes, inter alia,using a self-adhesive plaster with an organic light-emitting diode inorder to irradiate the cream arranged between the plaster and the skinsurface. The surrounding cancer tissue is then destroyed by thephotochemical reaction.

Generally, for kidney function testing in the prior art, recourse isregularly had to inulin as the gold standard. In this case, the inulinmeasurement is usually effected enzymatically, i.e. in a serum or urinesample taken. Noninvasive methods using fluorescence-marked inulinyielded ambiguous results (WO2001/85799). FITC sinistrin was establishedas the standard for fluorescence-based GFR determinations (WO2001/85799;Pill 2005, Anal Bioanal Chem 382: 59-64; Pill 2005, Europ J MedicinalChem 40: 1056-1061), wherein here as well the measurements werepredominantly effected in isolated samples.

However, these last-mentioned methods and devices known from the priorart are generally comparatively complex in respect of apparatus. Thus,systems based on sample collection, such as, for example, the systemdescribed in the publication by A. Pais et al., generally require atechnically complex microfluidic system, which can generally only berealized by means of corresponding microchannel structures. The othersystems described are also generally technically comparatively complex.Moreover, none of the systems described can be employed directly for ameasurement of a kidney function.

Consequently, one object of the present invention is to provide devicesand methods for determining organ functions, more particularly a kidneyfunction, which avoid the disadvantages of known devices and methods.More particularly, the intention is to provide a device which is simpleto handle and which also allows a simple, fast and nevertheless reliablemeasurement of the organ function without considerable interruption ofthe daily routine of the patients or at least in the context of anambulant treatment. This object is achieved by means of the inventionwith the features of the independent claims. Advantageous developmentsof the invention, which can be realized individually or in combination,are presented in the dependent claims.

In this case, a sensor plaster, a sensor system comprising the sensorplaster, a kit comprising the sensor plaster or the sensor system, usesof the sensor plaster, of the sensor system or of the kit, and a methodfor producing the sensor plaster, methods for the transcutaneousmeasurement of an organ function are proposed and uses of afluorescence-marked indicator substance for producing a diagnostic aidare proposed, which can optionally also be combined. Thus, by way ofexample, the method for the transcutaneous measurement of an organfunction can be carried out using one or more of the proposed devices,such that, for possible optional configurations of the method, referencemay be made to the description of the respective devices. Conversely,the devices can be designed to carry out a corresponding method. Thus,by way of example, in the devices, for example the sensor plaster, thesensor system or the kit, it is possible to provide one or a pluralityof data processing units designed in respect of programming, forexample, to perform partial steps of the predefined method in one of theembodiments described below.

One basic concept of the present invention consists in improving knownoptical devices and methods for determining the organ function, forexample the kidney function, by using small, integrated sensor plasters.Thus, a first aspect of the invention proposes a sensor plaster for thetranscutaneous measurement of an organ function, more particularly of akidney function, which can be used for example for the measurement ofthe renal clearance in accordance with the above description of theprior art. In this case, in the context of the present invention, aplaster is generally understood to mean a medical article comprising atleast one flexible carrier element having at least one adhesive surfacewhich can be applied, more particularly stuck, onto a body surface. Saidflexible carrier element can comprise for example a plastic, a textile,a ceramic, a paper or a combination of the aforementioned and/or othermaterials. The sensor plaster can therefore be configured inself-adhesive fashion and can comprise one or a plurality of adhesiveson the adhesive surface, for example. In a storage stage, the adhesivescan be protected by one or a plurality of protective films, for example,which can be pulled off, for example. This adhesive surface cantherefore enable a cohesive connection between the sensor plaster andthe body surface. In principle, however, alternatively or additionally,other types of connections between the adhesive surface and the bodysurface are also possible, for example force-locking connections. Thus,by way of example, the adhesive surface can be pressed onto the bodysurface by means of one or a plurality of clamping devices, for exampleby means of a finger clamp or some other type of mechanical device whichcan provide a press-on force for pressing the adhesive surface onto thebody surface. However, the use of self-adhesive adhesive surfaces isparticularly preferred. In principle, therefore, the adhesive surfacecan be configured as a self-adhesive adhesive surface. Alternatively oradditionally, however, in principle, adhesive surfaces are alsoconceivable in which one or a plurality of adhesives can subsequently beapplied in order to enable the connection. By way of example, by meansof an adhesive tube, skin-compatible adhesives can be applied to theadhesive surface in order then to stick the sensor plaster onto the bodysurface.

In this case, in principle, any desired surfaces of a body of a human oranimal patient come into consideration as a body surface. Examples thatmay be mentioned include skin surfaces, surfaces of fingernails ortoenails or other surfaces, more particularly surfaces exposed to theatmosphere. Generally, in this case, in the context of the presentinvention, the term “patient” is used for a human or an animal on whomor which one or a plurality of the proposed devices and/or methods areintended to be used, independently of whether said human or said animalis healthy or ill.

Furthermore, the sensor plaster comprises at least one radiation source.In this case, a radiation source is understood to be any device whichcan emit radiation. This can be, more particularly, electromagneticradiation, for example light in the visible and/or infrared and/orultraviolet spectral range and/or gamma radiation. Alternatively oradditionally, however, in principle, other types of radiation can alsobe used, for example streams of particles. By way of example alpha raysand/or beta rays can be mentioned in this connection. The radiationsource is correspondingly configured for generating radiation of thetype mentioned. Without restricting the possible further configurationsof the radiation, hereinafter the radiation is generally designated as“light”, the handling of the radiation as “optical system”, and theradiation source is described more particularly with reference to alight source. However, other configurations of the radiation source arealso possible, in principle, and it is also possible, for example, tocombine different types of radiation sources.

The radiation source can be, in particular, an integral constituent ofthe plaster, for example in the context of a layer construction of thesensor plaster. The radiation source is therefore designed to generateat least one interrogation light directly within the sensor plaster, incontrast to external generation of the interrogation light. In thisrespect, the sensor plaster differs for example from the fiber-opticconstruction in DE 100 23 051 A1, in which an external light source isused. Instead of an individual light source, it is also possible to usea plurality of light sources, for example redundant light sources foremitting one and the same wavelength, and/or a plurality of differentlight sources for emitting different wavelengths. Generally, the atleast one light source is intended to be designed to irradiate the bodysurface with at least one interrogation light.

In this case, in the context of the present invention, an interrogationlight is understood to be a light that can be used for the detection ofthe indicator substance in the sense of the above definition, whichlight excites the indicator substance inside a body tissue and/or a bodyfluid, for example with variable penetration depth, to bring about aperceptible response, more particularly an optically perceptibleresponse. This excitation can take place for example in such a way thata luminescence, more particularly a fluorescence and/or aphosphorescence, is excited in the indicator substance. Alternatively oradditionally, however, some other type of excitation can also takeplace, for example scattering of the light at an identical or shiftedwavelength. Generally, at least one response light is generated in thisresponse of the indicator substance.

In this case, the interrogation light is intended to be designed in sucha way that the desired response is excited in a targeted manner in theindicator substance. Accordingly, by way of example, a wavelength and/ora wavelength range of the interrogation light and/or some other propertyof the interrogation light can be adapted. This can be done directly bythe radiation source, for example, by virtue of said radiation sourcefor example already providing interrogation light having the desiredwavelength and/or in the desired wavelength range and/or by virtue of atleast one excitation filter additionally being used which filters outthe desired interrogation light from a primary light of the lightsource. In this case, it is particularly preferred if the sensor plasteris designed to perform fluorescence measurements on the indicatorsubstance. Accordingly, the interrogation light can be adapted to anexcitation range of this fluorescence of the indicator substance. If afluorescence of FITC is excited, for example, then it is possible to useinterrogation light in the spectral range around 480 nm, for exampleinterrogation light having a perceptible intensity in the range ofbetween 470 nm and 490 nm.

The sensor plaster furthermore comprises at least one detector designedto detect at least one response light incident from the direction of thebody surface. The response light can once again be light in the sense ofthe above definition. The detector, too, can in turn be an integralconstituent of the sensor plaster. The detector is therefore part of thesensor plaster, such that the response light is detected directly withinthe plaster, in contrast for example to the fiber-optic construction inDE 100 23 051 A1, in which an external detector has to be used.

The response light represents the optical response of the indicatorsubstance to the incidence of the interrogation light. Accordingly, thedetector and/or the detector in interaction with at least one responsefilter can be designed to detect in a targeted manner in the spectralrange of the response light. In this case, the detector and/or thedetector in interaction with the at least one response filter can bedesigned to suppress light outside the spectral range of the responselight. More particularly, the detector and/or the detector ininteraction with the at least one response filter can be designed tosuppress interrogation light. The interrogation light and the responselight can be configured, in particular, such that they are spectrallydifferent or spectrally shifted relative to one another, that is to saydifferent with regard to their spectral intensity distribution. Inparticular, the response light can be shifted toward longer wavelengthsin comparison with the interrogation light, which is generally the casefor example in a fluorescence measurement. By way of example, thespectral shift of a peak wavelength of the response light relative to apeak wavelength of the interrogation light can be between 10 nm and 100nm, more particularly between 30 nm and 50 nm, and particularlyapproximately 40 nm. The detector and/or the detector in interactionwith the at least one response filter can accordingly be designed todetect such response light. With the use of FITC, by way of example, thedetector and/or the detector in interaction with the at least oneresponse filter can be designed to detect response light having ameasurable intensity in the range of between 510 nm and 530 nm, inparticular at 520 nm.

The at least one radiation source, more particularly the at least onelight source, and the at least one detector are designed to irradiatethe body surface with the interrogation light and to detect at least oneresponse light incident from the direction of the body surface. Theradiation source and the detector are therefore optically connected tothe body surface in such a way that, through the body surface, forexample transcutaneously, the interrogation light can be radiated intothe body tissue or the body fluid and that, likewise through the bodysurface, for example once again transcutaneously, the response lightfrom the body tissue or the body fluid can be picked up by the detector.The proposed sensor plaster thus differs for example from lab-on-a-chipsystems, more particularly from microfluidic systems, which require asampling system and generally a complex microchannel structure.

The transcutaneous measurement according to the invention can beeffected, for example, by the radiation source and/or the detectorbearing directly and areally on the body surface. By way of example, theradiation source can comprise an emission surface which can be placedonto the body surface directly or with the interposition of one or aplurality of transparent layers. Accordingly, the at least one detectorcan comprise at least one sensor surface which can be applied to thebody surface for example directly or with the interposition of one or aplurality of transparent layers and via which the interrogation lightcan be emitted and the response light can be picked up.

In principle, numerous types of radiation sources can be used for theproposed sensor plaster. In this case, it is particularly preferred ifthe at least one radiation source is configured as a large-arearadiation source, that is to say as a radiation source having aradiation-emitting area, for example a light-emitting area, in contrastfor example to point light sources or point radiation sources. By way ofexample, large-area light sources having a light-emitting area of atleast 0.2 cm², preferably at least 0.5 cm² and particularly preferably 1cm² or more of light-emitting area can be used.

It is particularly preferred if the at least one radiation sourcecomprises at least one light source comprising an organic light-emittingmaterial, more particularly an organic light-emitting diode (OLED). Inthis case, an organic light-emitting material can be understood to mean,in principle, any organic material of natural and/or synthetic originwhich is able to emit light. Consequently, this term of OLED alsoencompasses bio-organic light-emitting diodes, for example. In thiscase, the generation of light in the organic material can be based onvarious mechanisms. Thus, by way of example, electroluminescence can beutilized, that is to say excitation of the organic material to emitlight by means of an electric current. However, other mechanisms arepossible, in principle, for example bioluminescence or other mechanisms.A combination of different mechanisms for generating light is alsoconceivable.

Alongside the organic light-emitting materials and the correspondinglight-emitting layers, further materials and/or functional layers can beprovided, for example charge carrier transport layers, barrier layers orsimilar materials and layers. In this case, purely organic componentscan be used, that is to say components which exclusively compriseorganic light-emitting materials and organic functional layers, orhybrid components can also be used, that is to say components whichcomprise both inorganic and organic light-emitting materials and/orfunctional layers. Both shall be encompassed hereinafter by the term ofan organic light-emitting diode.

With regard to the construction of organic light-emitting diodes,reference can be made, for example, to the constructions known from theprior art. By way of example, reference can be made to the organiclight-emitting diodes described in the above-cited publication by A.Pais et al., or the prior art concerning OLEDs cited in said document.

Organic materials used can be, for example, low molecular weight organicmaterials, that is to say monomers and/or oligomers, for example. As anexample of such low molecular weight substances, reference can likewisebe made to the substances used in the abovementioned publication by A.Pais et al. Alternatively or additionally, it is also possible to usepolymer materials, for example conjugated polymers. Typical polymermaterials of this type that can be mentioned, include, for example,fluorenes or polyphenylene vinylene derivatives (PPVs). Depending ontheir processing properties, the organic materials can be deposited forexample from the gas phase or else from the liquid phase, for example bymeans of a spin-on method or a printing process. Organic light-emittingdiodes are distinguished by the fact that large-area, homogeneouslyemitting light sources by means of which a large region of the bodysurface can be irradiated can be produced using this technology.

As an alternative or in addition to the complete or partialconfiguration of the light source as a light source comprising organiclight-emitting material, the at least one detector can also beconfigured wholly or partly as an at least partly organic detector.Thus, the at least one detector can comprise at least one detectorcomprising at least one organic semiconducting material, moreparticularly an organic photodetector (OPD).

With regard to organic photodetectors, too, which can be configured forexample wholly or partly as an organic solar cell and/or as an organicphotodiode, reference may largely be made to the literature. Thus, byway of example, with regard to possible configurations of the organicphotodetector, reference may once again be made to the above-citedpublication by A. Pais et al. Once again, it is possible to use fullyorganic components, or it is also possible to use hybrid componentscomprising a combination of organic and inorganic materials and/orfunctional layers. Once again, it is possible to use low molecularweight organic substances, that is to say monomers or oligomers, or,alternatively or additionally, once again also polymers. With regard topossible deposition methods or production methods for the organiccomponents, too, reference may at least largely be made to the abovedescription.

Analogously to the above-described advantages of organic light-emittingdiodes, OPDs also have similar advantages. Thus, with this technology,it is possible to produce large-area, thin photodetectors which,similarly to OLEDs can be integrated directly into the sensor plaster.By way of example, it is possible overall to use a layer technology inwhich the sensor plasters are constructed layer by layer. In this way,sensor plasters having at least two different layer planes can beproduced in a layer design. One of said layer planes can be, forexample, the at least one flexible carrier element, and others of saidlayer planes can comprise for example electronic components, for examplethe detector and/or the radiation source.

Alongside the at least one detector and the at least one radiationsource, the sensor plaster can comprise further elements. Thus, thesensor plaster can comprise for example at least one interface for dataexchange. Said data can be for example measurement results, for exampleintensities of the response light that was detected by the detector.Data already partly processed, for example filtered or partly orcompletely evaluated data, can also be transmitted via said interface.The interface can be configured as a wireless interface, in particular,and can comprise a radiofrequency coil, in particular. In this respect,a transponder technology known from the prior art can also be used, forexample, in order to initiate a measurement by means of the sensorplaster and/or to interrogate measurement data from the sensor plaster.Corresponding radiofrequency readers such as are known from RFIDtechnology (radiofrequency identification label technology), forexample, can be used for this purpose.

Furthermore, the sensor plaster can comprise at least one drivingelectronic unit. Said driving electronic unit can be configured, forexample, for driving the at least one radiation source and the at leastone detector, for example for starting an emission of the interrogationlight and/or for initiating a detection of the response light. For thispurpose, the driving electronic unit can comprise for examplecorresponding drivers for the detector and/or the radiation source. Atiming for a measurement can also be predefined, such that, for example,the driving electronic unit can predefine a specific time scheme for theradiation source and/or the detector, said time scheme allowing atemporal sequence of the emission of the interrogation light and thedetection of the response light. By way of example, the drivingelectronic unit can be designed to carry out or to control a temporallyresolved measurement of the sensor plaster. In this case, a measurementcomprises the emissions of at least one interrogation light, moreparticularly of at least one pulse of the interrogation light, and thedetection of at least one response light, more particularly of at leastone pulse of the response light. A temporally resolved measurement canaccordingly be understood to mean a measurement in which, in addition, atime of the detection of the response light also plays a part or isregistered. Thus, by way of example, for each value of the responselight, it is also possible to register the corresponding points in timeat which this value is recorded and/or it is possible for the responselight only to be recorded at specific points in time (gating). In thisway, by means of temporally resolved measurements, for example, it ispossible to obtain information about a depth from which the respectiveresponse light originates, for example by means of propagation timemeasurements. Alternatively or additionally, it is also possible to usecomplex measurement schemes in which, for example, the response light isdetected at a predefined point in time after the excitation by theinterrogation light.

Furthermore, the driving electronic unit, likewise alternatively oradditionally, can also be designed to carry out partial or completeprocessing of the measurement results. In particular, in this case it ispossible to process the signals recorded by the at least one detector,and optionally additional information such as, for example, timeinformation, for example the points in time at which the measurementsignals of the detector were recorded. The measurement values ormeasurement signals of the detector can be, for example, intensities ofthe response light and/or signals of electrical type which correlatewith said intensities. In this case, by way of example, complete orpartial processing of these signals can be effected, such that, forexample, filtering, smoothing, averaging or the like is already effectedin the driving electronic unit. Alternatively or additionally, anevaluation of these signals can also already be effected at least inpart, for example a determination of a waveform and/or of a half-lifeand/or a determination of an indicator substance concentrationcorresponding to these signals.

Partial or complete storage of the information in the sensor plaster,more particularly in the driving electronic unit, is also conceivable.Said information can comprise, for example, one or a plurality ofdetector signals or information derived therefrom, time information,information about the interrogation light, for example an intensity ofthe interrogation light, or combinations of said information and/orfurther information. In order to store the information, the sensorplaster, more particularly the driving electronic unit, can comprise forexample one or a plurality of data storage devices, more particularlyvolatile and/or nonvolatile data memories. Generally, the drivingelectronic unit can be configured wholly or partly using electricalcomponents, wherein one or a plurality of data processing units, forexample microprocessors and/or ASICs, can also be used.

The driving electronic unit can also be configured wholly or partly asorganic electronics. Thus the driving electronic unit can comprise forexample at least one organic component, that is to say a componentcomprising at least one organic material, more particularly an activeorganic material. By way of example, organic conductors and/orsemiconductors can be involved in this case. The organic component cancomprise for example an organic field effect transistor or simply anorganic conductor track.

Organic components of this type are known for example in the form ofpolymer electronics from DE 10 2004 048 864 A1. By way of example, it ispossible to produce organic field effect transistors using organicsemiconductor materials which can be part of the driving electronicunit. Simpler organic components can also be encompassed, such as, forexample, simple conductor tracks and/or connection contacts whichcomprise an organic conductive material, for example a conductivepolymer. The advantage of such driving electronic units constructedfully or partly using organic technology is once again that such drivingelectronic units can be produced in flat, small fashion andcost-effectively, such that they can also be used in disposable articlessuch as plasters, for example. Once again it is possible to use simpleand cost-effective layer designs for producing the driving electronicunit, for example printing techniques or the like. Generally, the sensorplaster can preferably be produced using a roll-to-roll technique inwhich numerous sensor plasters are produced as tape products.

Generally, it is particularly preferred if the driving electronic unitis configured such that it is robust and insusceptible to faults. Thus,by way of example, the driving electronic unit can be configured forenabling an adjustment and/or a calibration. By way of example,corresponding adjustment elements that enable an adjustment can beprovided in the driving electronic unit. By way of example, this caninvolve settable adjustment elements and/or elements which allowtrimming. This last can be effected for example by adjustment elementswhich can be set to the desired properties by means of a suitabletrimming process, for example a mechanical trimming method and/or alaser trimming. A trimming to variable properties is also possible, inprinciple, for example a trimming to a variable wavelength of theinterrogation light and/or of the response light. By way of example, alength of an adjustment element can be set by means of such a trimmingprocess.

Furthermore, the driving electronic unit can also be configured in adifferent way in order to enable repeatable measurement situations.Thus, the driving electronic unit can, for example, be configured inredundant fashion and contain one or a plurality of elements in multiplefashion, for example in order to compensate for a failure and/or amalfunction of one of these elements. Furthermore, it is also possibleto use calibrated components, for example calibrated amplifiers,calibrated analogue-to-digital converters, calibrated radiation sources,calibrated detectors or the like. Furthermore, it is possible to usefault-tolerant circuits, redundant circuits and/or compensatory circuitswhich can ensure a functionality. Furthermore, it is also possible toimplement test circuits which, for example, can internally storeparameters required during a calibration and make it possible for thesensor plaster, more particularly the driving electronic unit, itself tobe reconfigurable. In this way, it is possible to circumvent defectiveelements, for example, it is possible to set load resistances, or thelike.

Furthermore, the proposed sensor plaster can comprise at least onefilter element. Said filter element can be used in the beam path of theinterrogation light, and said filter element can also be used in thebeam path of the response light, and both possibilities can be realizedin combination. Thus, by way of example, it is possible to use at leastone filter element in the beam path of the response light, that is tosay at least one response filter, and/or at least one filter element inthe beam path of the interrogation light, that is to say at least oneexcitation filter. In this case, the at least one response filter andthe at least one excitation filter can have different spectralproperties, for example different peak transmissions. The at least oneexcitation filter and the at least one interrogation filter can beconfigured as separate components or can also be configured wholly orpartly as a common component. Furthermore, a configuration in which afilter element is provided only in one of said beam paths is alsoconceivable.

The at least one filter element can be utilized, for example, tospectrally separate the interrogation light from the response light. Byway of example, the interrogation light and the response light can beconfigured such that they are spectrally different, for examplespectrally shifted at least in part relative to one another. In thisway, by way of example, in front of the detector it is possible toemploy a filter element which at least partly prevents interrogationlight from passing into the detector and forming a disturbingmeasurement background and/or background there. Conversely, by way ofexample, alternatively or additionally, in front of the radiationsource, it is possible to employ a further filter element, which filtersout from the spectrum of the radiation source, which spectrum can beconfigured in broadband fashion, for example, only a specific spectralrange for the interrogation light. Various combinations are conceivable.

In principle, all filter elements having spectrally separatingproperties can be used as the filter element. Interference filters,dichroic mirrors, absorption filters or the like shall be mentioned hereas an example. It is particularly preferred if the at least one filterelement comprises at least one filter film, that is to say a thinflexible element. Said filter film can be adhesively bonded and/orprinted onto the remaining layers using layer technology, for example. Acombination of a plurality of filter films is also conceivable. The atleast one filter element can also be integrated wholly or partly in theradiation source and/or the detector. By way of example, it is possibleto use a radiation source with an integrated excitation filter and/or adetector with an integrated response filter.

Furthermore, the sensor plaster can comprise at least one imagingsystem, that is to say a system having at least one propertiesrefracting the light, that is to say the interrogation light and/or theresponse light. In this way, by way of example, the interrogation lightcan be focused onto a specific body region and/or the response lightfrom a body region can be focused onto the detector. In order to enablea configuration of the imaging system which is as simple as possible,saves as much space as possible and is as cost-effective as possible, itis particularly preferred if said imaging system comprises at least oneFresnel lens. Lenses of this type can be produced for example usingprinting and/or embossing technology, for example by the correspondingFresnel structures being embossed into a transparent plastic film. Thefilm embossed in this way can be applied, for example by adhesivebonding, onto the remaining layers of the sensor plaster beforehand orafter this treatment.

Furthermore, it is particularly preferred if the sensor plastercomprises at least one electrical energy storage device. Said at leastone electrical energy storage device makes it possible for the sensorplaster to be able to be operated autonomously, without having toproduce a wireless or wire-based connection for transmission ofelectrical energy to some other component. However, in principle, suchconnections are alternatively or additionally likewise possible. In thiscase, the at least one electrical energy storage device should beconfigured such that it is as flat as possible and preferably flexible.Accordingly, said at least one electrical energy storage device cancomprise a polymer battery, for example. Various configurations areconceivable.

As an alternative or in addition to the use of an electrical energystorage device, however, it is also conceivable for the electricalenergy required for the operation of the sensor plaster to be providedin some other way. Thus, by way of example, electrical energy can beradiated in externally, as generally takes place in the case of RFIDlabels, for example. Once again alternatively or additionally, energycan also be drawn from the surroundings in some other way, for examplein the form of heat and/or light. Such devices which draw energy in anyform from the surroundings of the sensor plaster and provide the energyas electrically usable energy for the operation of the sensor plasterare referred to hereinafter as an energy generating device. Accordingly,the sensor plaster can optionally comprise one or a plurality of suchenergy generating devices. Thus, by way of example, the sensor plastercan contain at least one of the following devices: a thermoelement, moreparticularly a Seebeck element and/or a Peltier element, for convertingthermal energy into electrical energy; a solar cell for converting lightinto electrical energy; a piezoelement for converting mechanical energy,more particularly from vibrations, into electrical energy. Combinationsof the aforementioned and/or other types of energy generating devicescan also be used.

If, by way of example, a solar cell is used as an energy generatingdevice and/or as part of said energy generating device, then said solarcell can for example in turn be constructed wholly or partly as anorganic solar cell. With regard to possible configurations, referencemay largely be made to the description of the detector. In contrast tothe detector, however, the solar cell is then arranged in such a waythat an active area of the solar cell does not face the body surface,for example the skin surface, but rather a direction from which, in astate in which the sensor plaster has been applied on the body surface,generally light incidence of ambient light, more particularlyinsolation, is to be expected. Thus, by way of example, on a side of thecarrier element which faces away from the active area of the radiationsource and/or of the detector, the sensor plaster can comprise one or aplurality of solar cells, more particularly organic solar cells, whichcan provide electrical energy to the sensor plaster applied to the bodysurface. This provision can be effected directly to the detector, to theradiation source, to the driving electronic unit or to other electricalcomponents of the sensor plaster, or the electrical energy can betemporarily stored, for example once again in one or a plurality ofelectrical energy storage devices, more particularly polymer batteries.Various configurations are conceivable.

As explained above, the sensor plaster overall is preferably producedwholly or partly in a layer design and comprises at least two differentlayer planes. Such a layer design enables an integrated construction ofhigh integration density. At the same time, cost-effective techniquescan be used. In particular, one or more of the following elements can beproduced wholly or partly in a layer design: an optical unit comprisingthe at least one radiation source and the at least one detector; anelectronic unit comprising the driving electronic unit; a communicationunit comprising the interface; a sensor module comprising the opticalunit, the electronic unit and the communication unit. Various techniquescan be used for producing a layer construction, for example laminationtechniques, embossing techniques, adhesive-bonding techniques, printingtechniques or combinations of the aforementioned and/or othertechniques. It is particularly preferred if the radiation source and/orthe detector are at least partly applied to the carrier element by meansof a printing technique. Accordingly, such a method for producing thesensor plaster is proposed. Other components of the sensor plaster, forexample one or more of the components mentioned above, can also beproduced by means of the printing technique. As an alternative or inaddition to the printing technique, which can comprise, for example,offset printing, screen printing, inkjet printing, pad printing,flexographic printing or a combination of the aforementioned and/orother types of printing, it is also possible to use other layertechnologies, for example stamping techniques, embossing techniques orthe like. In particular, the polymer electronics which can optionally beencompassed in the driving electronic unit, for example, can also beproduced in this way.

Alongside the sensor plaster, a sensor system for the transcutaneousmeasurement of an organ function, more particularly of a kidneyfunction, is furthermore proposed. The sensor system comprises at leastone sensor plaster in accordance with one or more of the embodimentsdescribed above. Furthermore, the sensor system comprises at least onereader designed to interact with the sensor plaster, wherein aninteraction with a plurality of sensor plasters is also possible. Inthis case, an interaction can generally be understood to mean afunctional interaction in which, for the purpose of the transcutaneousmeasurement of the organ function, control signals and/or informationare exchanged between the reader and the at least one sensor plaster. Inparticular, the reader can be designed to initiate a measurement of theorgan function by means of the sensor plaster. Alternatively oradditionally, the reader can also be designed, for example, to receiveinformation from the sensor plaster, for example the informationpresented above. The reader can be configured as a standing unit or,preferably, as a portable unit. In order to initiate the organ function,by way of example, at least one interface can be present, for example atleast one wireless and/or one wire-based interface by means of which,for example, a measurement, comprising the emission of interrogationlight and the detection of response light, can be started. The terminitiation should likewise be understood to encompass processes in whichan emission of interrogation light or a detection of response light iseffected permanently, for example, in which case only the respectiveother of said functions is initiated by the reader.

The reader can comprise for example a radiofrequency transmitter (RFtransmitter), for example a radiofrequency transmitter such as isusually used in RFID technology. Said radiofrequency transmitter can bedesigned to interact with the above-described optional radiofrequencycoil of the sensor plaster, for example by the frequencies of theseelements being tuned to one another. A unidirectional and/orbidirectional exchange of data and/or control commands can be effectedin this way. The radiofrequency transmitter can therefore constitute theinterface between the reader and the sensor plaster and/or form aconstituent of said interface.

The sensor system can also be configured in a more complex manner. Thus,by way of example, the sensor system can be designed to carry out aplurality of measurements at different points in time, wherein pointmeasurements or else continuous measurements can be encompassed. Thisimplementation of measurements at different points in time can, inparticular, also be effected automatically. Furthermore, the sensorsystem can be designed to determine a temporal concentration profile ofan indicator substance in a body tissue and/or a body fluid from themeasurement results of said measurements. In this case, the temporalconcentration profile can be understood to mean, for example, thecomplete or piecewise profile of the concentration, or it is alsopossible, alternatively or additionally, to determine other variables orparameters which characterize the concentration profile. As examples ofsuch variables, the half-life can be mentioned, although other variablescan also be used alternatively or additionally. Such variables arereferred to hereinafter generally as parameters derived from theconcentration profile.

In this case, the indicator substance can be configured as in the abovedescription of the prior art. In particular, the indicator substance cancomprise an endogenous and/or an exogenous indicator substance. In thisway, for example a clearance of the indicator substance, for example arenal clearance, can be determined by means of the proposed sensorsystem. In this case, the measurement results can directly reflect theconcentrations, or the measurement results can be variables thatcorrelate with the concentrations, for example fluorescence measurementresults, the intensity values of which can be proportional to theconcentration of the indicator substance in the body tissue and/or thebody fluid. Other configurations are also conceivable.

In order to determine the concentration profile of the indicatorsubstance, the measurement results can simply be stored, for example.For this purpose, by way of example, one or a plurality of volatileand/or nonvolatile data memories can be provided in the sensor plasterand/or the reader. By way of example, the measurement results can bestored as measurement value pairs in said memory, for example in thereader. Thus, each measurement value pair can comprise for example apoint in time of the measurement (for example indicated in arbitrary orabsolute time units) and one or a plurality of associated measurementvalues of the at least one detector, for example a measured photovoltageat a photodiode of the detector. This detection means, therefore, thatthe measurement results or the measurement value pairs can be compiledand provided for subsequent interrogation. Alternatively, oradditionally, however, it is also possible for the measurement resultsalready to be at least partly conditioned in the sensor system. Thus, byway of example, the sensor system can be designed to represent theconcentration profile, for example on one or a plurality of displays ofthe sensor system, more particularly of the reader. A user can thereforedirectly identify the profile. Alternatively or additionally, it is alsopossible for the measurement results already to be at least partlyanalyzed in the sensor system, such that, for example, eliminationhalf-lives, clearance or similar results which can be determined fromthe concentration profile can already be fully or partly determined inthe sensor system. For this purpose, the sensor system can comprise oneor a plurality of correspondingly designed data processing units forexample in the sensor plaster and/or the reader. The sensor system canalso interact with one or a plurality of further systems, for exampleone or a plurality of external data processing units. For this purpose,the sensor system can, for example, in turn have a wire-based and/orwireless interface by means of which, for example, the measurement dataor measurement results can be interrogated by means of a personalcomputer, a server or similar computer systems. In this way, afurther-reaching evaluation can take place in an external computersystem, or, for example, a treating physician can have access to themeasurement results.

Alongside the sensor plaster and the sensor system comprising the sensorplaster, a kit for the transcutaneous measurement of an organ functionis furthermore proposed. The organ function can be, more particularly,once again a kidney function. The kit comprises at least one sensorplaster in accordance with one or more of the embodiments describedabove. Alternatively or additionally, the kit can also comprise acomplete sensor system in accordance with one or more of the embodimentsdescribed above. In this respect, for the possible configurationsoverall reference may be made to the above description. The sensorplasters can be packaged for example individually or as a plurality, forexample in a primary package. The remaining constituents of the kit canbe contained, for example together with use instructions, in a furtherpackage, which can also comprise the sensor plasters.

Furthermore, the kit comprises at least one indicator substance. Saidindicator substance is intended to be able to be introduced in the bodyof a patient, for example by an injection, by being taken orally, by atransdermal administration or by a rectal administration. In thisrespect, the indicator substance is intended to have, in particular, thecorresponding compatibilities with the organism of a human or animalpatient whose organ function is intended to be measured.

Furthermore, the indicator substance is intended to be chosen in such away that its temporal concentration profile in the body of the human oranimal patient, more particularly in a body tissue and/or a body fluid,can be used or can serve as an indicator for the organ function. By wayof example, the body fluid can be blood, urine or preferablyinterstitial fluid.

An indicator substance whose concentration profile can be used as anindicator for the organ function should be understood to mean, inparticular, an indicator substance whose concentration is dependent atleast substantially, preferably completely, only on the organ functionto be observed. If, by way of example, a kidney function, moreparticularly a glomerular filtration rate, is examined, then theindicator substance used is preferably any desired substance which issubstantially exclusively filtered and is not secreted tabularly insignificant amounts, nor resorbed back from the primary urine, normetabolized in the body.

In this case, the indicator substance is intended to comprise at leastone marker designed to emit the at least one response light uponincidence of the at least one interrogation light from the radiationsource of the sensor plaster. As explained above, a plurality of activemechanisms for the emission of the response light can be consideredhere. In particular, these mechanisms can be luminescence, moreparticularly fluorescence and/or phosphorescence. However, othermechanisms are also possible, in principle, for example lightscattering, for example Raman and/or Stokes scattering. In principle,other mechanisms are also possible, for example absorption and/orreflection, preferably wavelength-dependent absorption and/orreflection. In this respect, the response light can comprise for examplea reflective, a transmitted or a scattered light beam or a combinationof such light beams. Alternatively or additionally, the response lightcan also comprise a fluorescent light and/or a phosphorescent light or aresponse light that arises in some other way during the interaction ofthe interrogation light with the marker.

In this case, the marker can likewise be configured in different ways.Thus, firstly, the indicator substance as a whole can be configured assuch a marker, such that, for example, spectroscopic properties, that isto say corresponding to one or more of the above-described activemechanisms for the interaction with the interrogation light, of theentire molecule or of all molecules of the indicator substance can beinterrogated by means of the interrogation light. Alternatively oradditionally, however, the indicator substance can also merely comprisethe marker as one of a plurality of constituents. Thus, by way ofexample, one or a plurality of marker radicals, marker groups or similarmarker constituents can be coupled to the indicator substance by meansof bonding. By way of example, this can involve covalent bonding,complex bonding, ionic bonding or else simple bonding by means ofVan-der-Waals forces. The marker can comprise for example a fluorescentmolecule, for example fluorescein isothiocyanate (FTTC) described above.

The indicator substance according to the invention is thereforepreferably a fluorescent-marked indicator substance. The latterpreferably has a structure according to the general formula (I):

P-F  (formula I)

where P is a polyol; andwhere F is a marker having optically measurable properties, moreparticularly a fluorescent and/or phosphorescent marker.

Polyols for the indicator substance preferably comprise polyethyleneglycol, ethylene glycol, propylene glycol, glycerol, mannitol, sorbitol,hexitols, pentitols, tetritols, inositols, mannose, aldoses, lactose,cellobiose, gentiobiose, β-alkyl glycosides, deoxy sugar, β-alkyl uronicacids, fucose, deoxy sugar alcohols, fructose, and respectivederivatives, wherein the polyol is present as deoxyamino sugar alcohol.The polyol is preferably a polysaccharide, particularly preferablyinulin or sinistrin and more particularly an inulin or mixture ofinulins comprising from 3 to 20, preferably 11 to 15 or 3 to 8, fructoseunits.

The marker is preferably selected from the group consisting of:fluorescein dyes, cyanine dyes, naphthyl amide dyes, coumarin dyes,xanthene dyes, thioxanthene dyes, naphtholactone dyes, azlactone dyes,methine dyes, oxazine dyes, thiazine dyes. F is preferably a fluoresceindye, particularly preferably fluorescein.

The fluorescent marker can preferably be bonded to the polysaccharide bymeans of a coupling group. Suitable coupling groups and couplingreactions are known to the person skilled in the art. Particularlypreferably, the coupling group is selected from the group consisting of:thiourea group (—N—CS—N—), thiocarbamate group (—N—CS—O—), carbamate(urethane) group (—N—CO—O—), ether group (—O—), thioether group (—S—),ester group (—CO—O—), amide group (—CO—N—), thioester group (—CS—O—),thioamide group (—CS—N—), amino alkyl group (—CO—N—(CH₂)n-O—) where n=2to 5, secondary amine group (—NH—). In particular, the fluorescentmarker is present as fluorescein isothiocyanate (FITC).

Such substances can be used more particularly for the kidney functionmeasurement, as well as other indicator substances which are eliminatedexclusively via the urinary tract in the human body. The use offluorescence-marked polysaccharides and/or cyclosaccharides such as, forexample, sinistrins and/or fructosans which are marked with FITC, forexample, is particularly preferred. For the production of such markedpolysaccharides and/or cyclosaccharides, reference may be made forexample to the above prior art, for example WO2001/85799 orWO2006/32441.

Alongside the sensor plaster, the sensor system or the kit, in each casein one or more of the embodiments described above, the use of one ormore of these devices for a transcutaneous measurement of an organfunction is furthermore proposed. More particularly, this can involve akidney function, more particularly a glomerular filtration rate.

A method for the transcutaneous measurement of an organ function iscorrespondingly proposed, more particularly of a kidney function. Thismethod can be carried out more particularly using a sensor plasterand/or a sensor system and/or a kit in accordance with one or more ofthe embodiments described above, such that, for possible configurationsof the method, reference may largely be made to the above description.

The method comprises the following steps, which preferably, but notnecessarily, are carried out in the order presented below. Additionalmethod steps (not presented) can also be carried out and/or individualor a plurality of the method steps can be carried out temporally inparallel, in a temporally overlapping manner or else repeatedly.

In a first method step, a sensor plaster is applied, more particularlystuck, onto a body surface. The sensor plaster comprises at least oneradiation source, preferably as an integral constituent, wherein theradiation source is designed to irradiate the body surface with at leastone interrogation light. The sensor plaster furthermore comprises adetector, preferably likewise as an integral constituent, which isdesigned to detect at least one response light incident from thedirection of the body surface.

In a further method step, at least two temporally delimited measurementsat different points in time and/or at least one continuous measurementover a time period are carried out, wherein the response light isdetected at the different points in time and/or over the time period. Inthis respect, it is possible to form for example once again, asdescribed above, measurement value pairs in which one point in time isassigned one or a plurality of measurement values of the detector, forexample corresponding sensor signals. The detection can likewise beeffected in accordance with the above description, such that, forexample, storage and/or provision of said measurement value pairs can beeffected.

In a third method step, a temporal profile of a concentration of anindicator substance is then deduced from a temporal profile of theresponse light. In this case, the temporal profile of the response lightmay be known continuously or in a pointwise manner Thus, as explainedabove, the temporal profile can be measured continuously, for example.Alternatively or additionally, however, an extrapolation and/orinterpolation of individual measurement values can also be effected, forexample by adaptation of one or a plurality of the measurement curves.By way of example, said adaptation can already be fully or partlyeffected in the sensor plaster and/or in a reader of the sensor system.Other configurations are also conceivable, for example subsequentexternal evaluation in a separate computer system.

As explained above, the method is intended to be performed, inparticular, in such a way that the response light correlates with theconcentration of the indicator substance. In this case, it is possibleto utilize for example the above-explained interaction mechanismsbetween the interrogation light and the indicator substance and/or amarker of the indicator substance, for example a fluorescent mechanism.Since, for example from calibration measurements and/or empirical orsemi-empirical or theoretical considerations, a relationship between theconcentration of the indicator substance and the response light, forexample an intensity of the response light and/or a detector signal ofthe detector, is known or can be determined, this conclusion drawn fromthe temporal profile of the response light about the concentration ofthe indicator substance is easy to realize for the person skilled in theart. By way of example, this conversion into the concentration of theindicator substance can be effected in arbitrary units, such that, forexample, the intensity of the response light can be used directly as ameasure of the indicator substance. Alternatively or additionally,however, some other type of conversion can also be effected, for exampleby means of one or a plurality of stored conversion curves, conversionalgorithms or conversion tables which, for example, can be used in oneor a plurality of data processing units. Thus, by way of example, thisconversion can be effected fully or partly in a data processing unit ofthe sensor plaster and/or in a data processing unit of the reader and/orin a further, external data processing unit.

As explained above, the indicator substance can be an endogenous orexogenous indicator substance. In this respect, this indicator substancecan, for example, be present anyway in the body of the human or animalpatient and/or can be artificially increased in its concentration for ashort time by artificial uptake of the indicator substance, for exampleby being taken orally, by rectal administration or by injection, inorder then to terminate the supply. Alternatively or additionally, it isalso possible, for example, to regulate a supply of the indicatorsubstance in such a way that the temporal profile of the concentrationof the indicator substance is substantially constant, wherein thecorresponding organ function can be deduced from the requiredreplenishment rate, for example measured in quantitative units or massunits per unit time. That, too, is intended to be encompassed by theconcept according to the invention that the temporal profile of theconcentration of the indicator substance is deduced from the temporalprofile of the response light. Various other measurement methods areconceivable. The supply of the indicator substance can correspondinglybe part of the proposed method.

Overall, the proposed devices and methods have a large number ofadvantages, which can be realized individually or in combination, bycomparison with known devices or methods of this type. Thus, by way ofexample, the sensor plaster can be configured as a printable,intelligent sensor sticking plaster based on electronics. It istherefore possible to realize a sensor plaster with low production costssince, for example, printing methods with large-scale printing machinescan be used. In this case, it is also possible to use inexpensive rawmaterials such as, for example, cost-effective organic polymers for thedetector and/or the radiation source or light source and/or otherconstituents of the electronics, for example of the evaluationelectronics.

Furthermore, for the detector, the data processing, the storage and theinterface or combinations of these and/or other elements, it is possibleto use standard elements which can also be used again in otherconfigurations. In this respect, it is possible to realize a modularsystem, which can likewise in turn lead to reduced production costs,reduced stock-keeping costs and thus overall to a lowering of costs.

The sensor plaster can thus be configured, in particular, as a highlyintegrated sticking plaster. The dimensions of this sticking plaster cancorrespond to the dimensions of customary sticking plasters, that is tosay for example in the range from 5 to 100 mm×5 to 100 mm. The sensorplaster can be composed of an optical unit in the form of the radiationsource, for example a light-emitting diode, a laser or the like, and oneor a plurality of detectors, which can likewise be assigned to theoptical unit. Said detector can comprise, as explained above, aphotodiode and/or a solar cell, for example. The optical unit comprisingthe radiation source and the detector can be embodied as an independentunit, for example, which can be applied for example also in a spatiallycontinuous fashion on the sensor plaster. This optical unit can becombined for example with one or a plurality of filter films and/or withoptical imaging systems produced by pressing technology or printingtechniques, for example Fresnel lenses. In this way it is possible toproduce an optical unit which operates reliably, is cost-effective andhas an extremely small volume and has a high degree of integration.

Alongside the optical unit, an electronic unit can be provided, whichcan comprise, for example, the evaluation electronics described above.The latter can comprise, for example, suitable amplifiers, converters(for example A/D converters), controllers, storage elements orcombinations of the aforementioned and/or other components.

As an alternative or in addition to the electronic unit and alongsidethe optical unit, the sensor plaster can furthermore comprise one or aplurality of communication units. By way of example, this can involve,as explained above, an RFID-based communication unit. The latter cancomprise, for example, one or a plurality of radiofrequency coils. Thecommunication unit can interact functionally with the optical electronicunit and/or the optical unit.

Alongside the optical unit and the optional electronic unit and/or theoptional communication unit, the sensor plaster can comprise furtherelements such as, for example, the electrical energy storage deviceand/or the energy generating device, such as the solar cell, forexample. Other elements can also be encompassed, for example displayelements or the like, which makes it possible for a user to exchangeinformation and/or control signals with the sensor plaster.

The construction of the sensor plaster according to the invention can beimplemented in a comparatively simple manner. Thus, in each case atleast one detector, for example at least one solar cell, and at leastone radiation source, for example at least one OLED, can be printed onfor example alongside the at least one adhesive surface, for examplehaving two adhesive regions, in the center of the sensor plaster. Ineach case suitable filter films can be situated in front of said opticalelements, which filter films can prevent, for example, interrogationlight from being concomitantly detected by the detector to aconsiderable extent. The driving electronic unit for the optical unitcomprising the detector and the radiation source can be situatedalongside and/or behind said optical unit. Said driving electronic unit,as explained above, can likewise once again be configured as acost-effective printed driving electronic unit and can contain a drivingsystem for the detector and/or the radiation source. A device fordigitizing the measurement signals, for example the signals generated bythe detector, can also be provided. Furthermore, alternatively oradditionally, it is also possible to provide one or a plurality ofstorage elements and/or a control electronic unit for the read-out, forexample by means of radiofrequency signals.

Likewise using layer technology it is possible to produce the interface,for example with the radiofrequency coil. The latter can, for example,in turn be produced in an overlying layer plane and can generateradiofrequency signals, which can then be read out. By way of example,the reader can comprise a conventional RFID reader for reading out theradiofrequency signals. This information can then be transmitted by thereader, for example into a suitable database, which can be part of thereader or part of a separate unit. From said database, for example afurther evaluation of the measurement signals or measurement results canthen be effected later.

The energy required for picking up the measurement signals can beprovided wholly or partly by the optional energy storage device, which,for example, can likewise be integrated into the sensor plaster. By wayof example, said electrical energy storage device can in turn beconstructed fully or partly using polymer technology, for example fullyor partly as a polymer battery. By way of example, a printing techniquecan once again be used for applying said polymer battery. Alternativelyor additionally, other types of energy storage devices can also be used,for example conventional thin-film energy storage devices. Once againalternatively or additionally, however, it is also possible to use otherenergy sources, for example energy sources which are mounted externallyand which can be connected to the sensor plaster via one or a pluralityof interfaces. Thus, for example, a wireless transmission of energy tothe sensor plaster can be effected, and/or a transmission by means of apower supply cable (which can be attached to the sensor plaster, forexample).

The carrier material or the at least one carrier element of the sensorplaster can perform further tasks alongside provision of the at leastone adhesive area for sticking onto the body surface of the human oranimal patient. Thus, the carrier material can be chosen, for example,in such a way that it has substantially light-tight properties, suchthat, for example, no disturbing stray light, for example ambient light,can pass through the carrier material to the detector and/or to the bodysurface to be irradiated with the interrogation light. A disturbingstray light background can be suppressed in this way. Furthermore, thesensor plaster, for example the carrier element and for example theadhesive areas thereof, can be configured in such a way that no light,for example ambient light, can penetrate laterally. By way of example,this can be effected by virtue of the fact that the adhesive areasenclose the optical unit, that is to say the detector and/or theradiation source, completely in the plane of the body surface.Penetration of stray light and/or ambient light can likewise beprevented in this way. Furthermore, the adhesive used and/or othermaterials of the sensor plaster can also be configured in light-tightfashion, that is to say in such a way that they are configured such thatthey are largely nontransparent or have low transparency to light in thespectral range of the interrogation light and/or the response light.

The sensor system can be put into operation, for example, by aradiofrequency pulse, for example emitted by the reader, initiating oractivating the sensor plaster, for example a driving electronic unit ofthe sensor plaster. The sensor plaster can thereby be excited to recordmeasurement data. Said measurement data can be digitized, for example,and entered into one or a plurality of storage elements. As describedabove, said one or plurality of storage elements can be configured asmeasurement value memories, for example as volatile and/or nonvolatilememory, for example as flash-type memory. Said at least one storageelement can, for example, likewise be contained in the sensor plaster.In the latter case, these data can then be read out for example by thereader, for example once again by means of radiofrequency technology.Alternatively or additionally, at least partial data processing can alsoalready be effected on the sensor plaster, such that data that havealready been at least partly processed can be forwarded to the reader.Once again as an alternative, it is also possible for completely rawdata, for example data generated directly by the detector, already to beforwarded to the reader in order to be stored there in one or aplurality of storage elements. Various combinations are conceivable.

In the configuration of the detector and/or the radiation source and/orthe evaluation electronics or other electronic components of the sensorplaster, it is possible, as already mentioned in part above, to designthe components individually or in groups in such a way that repeatablemeasurement situations are possible. Thus, it is preferred particularly,as explained above, if calibrated radiation sources, for examplecalibrated light-emitting diodes and/or lasers, are used. Alternativelyor additionally, correspondingly calibrated detectors can also be used.Furthermore, likewise alternatively or additionally, further electroniccomponents can also be configured as calibrated components. By way ofexample, calibrated amplifiers and/or A/D converters can be used. Inorder that the measurement situation is further made repeatable, it isalso possible to use fault-tolerant and/or redundant electrical circuitswhich, for example, can also be configured in a compensatory fashion.The functionality can be ensured in this way. Furthermore, it is alsopossible to use test circuits in order to internally store the requiredparameters during the calibration and to allow the system to configureitself accordingly. In this way it is possible, for example, tocircumvent defective elements, it is possible to set load resistances,or the like. The evaluation circuit can optionally comprise one or aplurality of such test circuits. Overall, the proposed devices can thusbe configured in a manner insensitive to interference and enablereliable and reproducible measurements.

In principle, the present invention also relates to the use of afluorescence-marked indicator substance for the production of adiagnostic aid for determining the glomerular filtration rate (GFR).

The fluorescence-marked indicator substance used according to theinvention in this connection is preferably a polysaccharide,particularly preferably inulin or sinistrin and, more particularly, amixture of inulins comprising from 3 to 20, preferably 11 to 15 or 3 to8, fructose units, wherein the inulins are coupled to a fluorescentmarker. The fluorescent marker is preferably selected from the groupconsisting of: fluorescein dyes, cyanine dyes, naphthyl amide dyes,coumarin dyes, xanthene dyes, thioxanthene dyes, naphtholactone dyes,azlactone dyes, methine dyes, oxazine dyes, thiazine dyes. F ispreferably a fluorescein dye, particularly preferably fluorescein.

The fluorescent marker can preferably be bonded to the polysaccharide bymeans of a coupling group. Suitable coupling groups and couplingreactions are known to the person skilled in the art. Particularlypreferably, the coupling group is selected from the group consisting of:thiourea group (—N—CS—N—), thiocarbamate group (—N—CS—O—), carbamate(urethane) group (—N—CO—O—), ether group (—O—), thioether group (—S—),ester group (—CO—O—), amide group (—CO—N—), thioester group (—CS—O—),thioamide group (—CS—N—), amino alkyl group (—CO—N—(CH₂)n-O—) where n=2to 5, secondary amine group (—NH—). In particular, the fluorescentmarker is present as fluorescein isothiocyanate (FITC).

Preferably, the inulin mixture can be obtained by enzymatic digestionand subsequent chromatographic separation of naturally occurring inulin.By means of enzymatic digestion using a β-glucosidase, preferablyinulinase [E.C.: 3.2.1.7], and the subsequent chromatography, mixturesof inulin having a degree of polymerization (i.e. number of saccharidemonomer units in the polysaccharide) of between 3 and 20 and preferablybetween 3 and 8 or 11 and 15 can be provided in a targeted manner.Depending on the constitution of the starting material, correspondinginulin mixtures can also be obtained just by chromatographic separation.

The fluorescence-marked indicator substance is formulated as adiagnostic aid according to the invention. In this case, a definedquantity sufficient to generate a detectable fluorescent signal afteradministration is dissolved in a physiologically tolerated solvent, e.g.water or aqueous salt solutions, PBS, etc., and if appropriate admixedwith physiologically tolerated auxiliaries, e.g. stabilizers. It goeswithout saying that the quantity of fluorescence-marked indicatorsubstance can differ depending on the use of the diagnostic aid anddepending on the subject to be examined. Factors that can play a part inthis connection are body weight, age, sex, type and extent of the kidneydysfunction or presumed kidney dysfunction, and/or medical history. Adiagnostic aid within the meaning of the present invention can finallyalso contain indications concerning the type, duration, extent and sideeffects of the use, which can be enclosed in the form of an instructionleaflet or in electronic form, e.g. on a data carrier. Furthermore, theinstruction leaflet or the data carrier can contain indications thatallow an interpretation of the GFR.

The term of glomerular filtration rate (GFR) has already been defined indetail elsewhere in the description. The determination of the GFRpreferably serves, according to the invention, for diagnosing existingkidney dysfunctions, for determining the risk of future progression ofthe kidney dysfunctions, for monitoring in the case of diseases,therapeutic interventions or therapies which can cause kidneydysfunctions, or for determining the individual dose for medicamentsthat are excreted via the kidney. Kidney dysfunctions should beunderstood to mean all pathological alterations of the kidney functionwhich result in a changed and preferably decreased, but also increased,GFR. These preferably include chronic kidney dysfunctions and acutekidney failure, but also hyperfiltration and e.g. in the case of poorlycontrolled diabetes mellitus. However, kidney dysfunctions can also bebrought about as secondary disturbances resulting from other diseases.Thus, kidney dysfunctions can also occur in the presence ofcardiovascular diseases or when there is a predisposition for theoccurrence of cardiovascular diseases and in the case of diabetesmellitus order renalis.

Depending on the purpose of determining the GFR, the diagnostic aid canbe administered as a bolus or by infusion. Accordingly, differentaspects of the GFR can be measured such as the so-called inputclearance, infusion clearance or bolus clearance.

Advantageously, the diagnostic aids disclosed here are suitable for thenoninvasive, transcutaneous measurement of the GFR. Thefluorescence-marked indicator substances penetrate after administrationinto the interstitial space, where a nondisruptive determination of thefluorescence after excitation is possible. The determination ispreferably effected using a device as disclosed elsewhere in thedescription, but can also be effected using other methods and devicesknown in the prior art for the quantification of fluorescent substances.A further advantage of the diagnostic aids used according to theinvention is that the fluorescent-marked indicator substance consists ofa defined mixture of polysaccharides, more particularly inulins. Thisallows a standardization of the GFR determination, which was problematicpreviously since although inulin is the gold standard for determiningthe GFR, it has disadvantages with regard to standardization on accountof a changing composition. Through the use of smaller polymers it isadditionally possible to increase the solubility in particular in waterand aqueous solutions. Precipitation problems, which also consequentlylead to clinical side effects, can likewise be avoided. By virtue of theincreased solubility, it is additionally possible to administer smallervolumes as diagnostic aid, which additionally increases thebiocompatibility. Through the use of smaller polymers, moreover, anoptimum degree of marking with the fluorescence marker relative to theoverall molecule is also achieved, which makes it possible to reduce thequantity of fluorescence-marked indicator substance in the diagnosticaid. Therefore, less indicator substance has to be administered, sincethe fluorescence marking occurs more frequently in the same volume.Finally, by virtue of the ratio of marker to polymer in thefluorescence-marked indicator substances that are to be used as adiagnostic aid according to the invention, the lipophilic properties ofsaid substances is also increased. As a result, the renal excretion rateis reduced and the half-life in the organism is increased.

Finally, the invention also relates to a method for determining theglomerular filtration rate (GFR), comprising the following steps:

-   a. administering a fluorescence-marked indicator substance,    preferably a mixture of inulins, as explained above, to a subject;-   b. measuring the fluorescence noninvasively on the body surface; and-   c. determining the GFR on the basis of the measurement values from    step b.

The method according to the invention is preferably carried outnoninvasively. The device according to the invention can be used forthis purpose. However, other systems known in the prior art forfluorescence measurement can also be used. As has already beenexplained, the GFR can be determined—depending on the further purpose ofuse—as input clearance, infusion clearance or bolus clearance.Accordingly, the administration can be effected as bolus provision, asinfusion or as a mixed form. The measurement can also be a singlemeasurement (determination of the fluorescence at one specific point intime) or a repeated measurement (determination of the fluorescence at aplurality of points in time for profile representation).

The GFR can be determined in relative or absolute fashion. Within themeaning of the present invention, relative determination should beunderstood as the determination of a change, i.e. of an increase ordecrease in the GFR. This can, if appropriate, also be expressed as apercentage change from an initial value. The determination of theabsolute GFR presupposes that firstly a calibration for the indicatorsubstance is carried out, which allows a specific concentration ofindicator substance in the blood, plasma or serum to be assigned to aspecific measured fluorescence value. On the basis of thisconcentration, the GFR can then be calculated using the formulae knownin the prior art.

The method can be partly automated. As already mentioned, the devices ofthe present invention can be used for the measurement. The evaluationand calculation of the GFR can be effected in a computer-aided manner.

In one preferred embodiment of this method, a diagnosis can also be madeon the basis of the GFR. A statistically significantly reduced GFR ispreferably an indicator for a kidney dysfunction or a predispositiontherefor. A statistically significant reduction of the GFR can also bean indicator for lowering the dosage of medicaments that are excretedvia the kidney. Conversely, an increased GFR can be an indicator that nokidney dysfunction or predisposition therefor is present. The increasedGFR also indicates the need to increase the dosage of medicaments thatare excreted via the kidney. Such diagnostic evaluations of the GFRdetermined by the method according to the invention can, of course, alsobe effected in an automated manner, e.g. by using a diagnostic algorithmimplemented on a computer.

The sensor plaster or sensor system according to the invention and thekit according to the invention can also be used for the transcutaneousmeasurement of an organ function, which presuppose a functioning barrierbetween blood vessel system and extravasal spaces. Preferably, it ispossible to use sensor plasters, sensor systems or kit for thetranscutaneous measurement of the intestinal wall barrier function orthe blood-brain barrier function. In this case, the barrier function canbe determined by determining the increase or decrease influorescence-marked indicator substance in the blood. It goes withoutsaying here that an intensified decrease in the fluorescence-markedindicator substance in the blood will occur in the case of a disturbedbarrier function. Conversely, an increase in fluorescence in the bloodis possible after oral administration of the fluorescence-markedindicator substance in the presence of a barrier disorder.

The invention therefore also relates to a method for the transcutaneousmeasurement of the intestinal wall barrier function or of theblood-brain barrier function, more particularly using a sensor plaster(116) as claimed in any of the preceding embodiments relating to asensor plaster (116) and/or a sensor system (114) as claimed in any ofthe preceding embodiments relating to a sensor system (114) and/or a kit(110) as claimed in any of the preceding embodiments relating to a kit(110), wherein the method comprises the following steps:

-   -   a sensor plaster (116) is applied, more particularly stuck, onto        a body surface, wherein the sensor plaster (116) comprises at        least one radiation source, wherein the radiation source is        designed to irradiate the body surface with at least one        interrogation light (162), wherein the sensor plaster (116)        furthermore comprises a detector (146), wherein the detector        (146) is designed to detect at least one response light (176)        incident from the direction of the body surface;    -   at least two temporally delimited measurements at different        points in time and/or at least one continuous measurement over a        time period are carried out, wherein the response light (176) is        detected at the different points in time and/or over the time        period; and    -   a temporal profile of a concentration of an indicator substance        (112) is deduced from a temporal profile of the response light        (176).

Preferably, the mixture according to the invention of inulins or an FITCinulin or an FITC sinistrin is used in the abovementioned methods oruses.

However, the invention also relates to the use of a fluorescence-markedindicator substance and preferably of the mixture according to theinvention of inulins or of an FITC inulin or of an FITC sinistrin forthe production of a diagnostic aid for diagnosing dysfunctions of theintestinal wall barrier or of the blood-brain barrier.

In this case, the occurrence of dysfunctions of the intestinal wallbarrier is preferably connected with the occurrence of Crohn's diseaseor ulcerative colitis, such that the abovementioned uses and methods canbe used, in principle, for diagnosing these diseases.

Dysfunctions of the blood-brain barrier occur in connection with varioushereditary diseases, but can also be connected with other diseases, e.g.neurodegenerative diseases, inflammations of the CNS or stroke.Hereditary diseases with disorders of the barrier function of theblood-brain barrier that are taken into consideration preferably includeGLUT1 deficiency syndrome, hereditary folate malabsorption orbiotin-responsive basal ganglia disease.

Sensor plasters, sensor systems or kit according to the presentinvention can also be used for determining the pancreas function. Inthis case, the function of the arylesterases of the pancreas isdetermined by transcutaneous measurement of the increase in fluorescencein the blood. In this case, the fluorescence originates fromenzymatically released fluorescein, for example, which originates fromfluorescein dilaurate which can be administered as substart of thearylesterases to the subject to be examined. Similar substrates that canbe used for determining the pancreas function includefluorescence-marked triglyceride analogues or a nitrophenyl ester of afluorescence-marked alkylphosphonatc. A more detailed description ofsuch substrates is found in Scholze 1999, Analytical Biochemistry276:72-80 or Negre-Salvayre 1990, Lipids 25 (8): 428-434. Reference ishereby expressly made to the substrates disclosed therein.

Consequently, the invention also relates to a method for thetranscutaneous measurement of the pancreas function, more particularlyusing a sensor plaster (116) as claimed in any of the precedingembodiments relating to a sensor plaster (116) and/or a sensor system(114) as claimed in any of the preceding embodiments relating to asensor system (114) and/or a kit (110) as claimed in any of thepreceding embodiments relating to a kit (110), wherein the methodcomprises the following steps:

-   -   a sensor plaster (116) is applied, more particularly stuck, onto        a body surface, wherein the sensor plaster (116) comprises at        least one radiation source, wherein the radiation source is        designed to irradiate the body surface with at least one        interrogation light (162), wherein the sensor plaster (116)        furthermore comprises a detector (146), wherein the detector        (146) is designed to detect at least one response light (176)        incident from the direction of the body surface;    -   at least two temporally delimited measurements at different        points in time and/or at least one continuous measurement over a        time period are carried out, wherein the response light (176) is        detected at the different points in time and/or over the time        period;    -   a temporal profile of a concentration of an indicator substance        (112) is deduced from a temporal profile of the response light        (176), wherein the indicator substance is fluorescein dilaurate,        a fluorescence-marked triglyceride analogue or a nitrophenyl        ester of a fluorescence-marked alkyl phosphonate.

EXEMPLARY EMBODIMENTS

Further details and features of the invention will become apparent fromthe following description of preferred exemplary embodiments. Theexemplary embodiments are illustrated schematically in the figures. Inthis case, identical reference symbols designate elements which areidentical or functionally identical or correspond to one another interms of their functions. The invention is not restricted to theexemplary embodiments.

Specifically in the figures:

FIG. 1 shows an exemplary embodiment of a sensor system and kitaccording to the invention for the transcutaneous measurement of anorgan function;

FIGS. 2A and 2B show an exemplary embodiment of a sensor plasteraccording to the invention in different illustrations;

FIG. 3 shows an exemplary embodiment of an organic light-emitting diodethat can be used in the sensor plaster;

FIG. 4 shows an exemplary embodiment of an organic solar cell that canbe used in the sensor plaster;

FIG. 5 shows a flowchart of a possible exemplary embodiment of a methodaccording to the invention for the transcutaneous measurement of anorgan function;

FIGS. 6A to 6D show a detection of fluorescence-marked inulin fractionsin the interstitial tissue;

FIGS. 7A to 7D show clearance experiments with F5 and F10 inulinfractions and sinistrin.

EXAMPLE 1: MEASUREMENT SET-UPS

An exemplary embodiment of a kit 110 according to the invention for thetranscutaneous measurement of an organ function is illustrated highlyschematically in FIG. 1. In this exemplary embodiment, the kit 110comprises an indicator substance 112, which here is illustratedsymbolically as the content of a syringe. As an alternative or inaddition to an injection of said indicator substance 112, however, othertypes of administration are also taken into consideration, for exampleoral, transdermal or rectal administrations. Furthermore, it is alsopossible to have recourse to endogenous indicator substances.Accordingly, the kit 110 can comprise suitable forms of administrationfor said indicator substance 112, for example syringes, ampoules,tablets, bags, small tubes or the like.

Alongside the indicator substance 112, the kit 110 in the exemplaryembodiment illustrated comprises a sensor system 114 for thetranscutaneous measurement of an organ function. The sensor system 114comprises a sensor plaster 116 for the transcutaneous measurement of anorgan function, said sensor plaster merely being indicated symbolicallyin FIG. 1. Furthermore, the sensor system 114 comprises a reader 118,which is likewise shown highly schematically. The reader 118 cancomprise one or a plurality of input and output means, for example,which are illustrated symbolically in the form of operating elements 120in FIG. 1. Furthermore, the reader 118 can comprise one or a pluralityof indicator elements 122, for example one or a plurality of displays,acoustic indicator elements or the like, for example in order to conveymeasurement results or other information to a user.

Furthermore, the reader 118 can comprise one or a plurality ofinterfaces 124, for example a radiofrequency interface 126, forcommunication with the sensor plaster 116. Alternatively oradditionally, further interfaces 124 can be provided, for examplewire-based interfaces, for example likewise for communication with thesensor plaster 116 and/or with further electronic equipment, for examplean external computer system. Wireless communication by means ofradiofrequency electromagnetic radiation is designated symbolically bythe reference numeral 128 in FIG. 1. As indicated in FIG. 1, thiscommunication 128 can take place bidirectionally or can also take placejust unidirectionally.

Furthermore, as indicated in FIG. 1, the reader 118 can comprise adriving and evaluation electronic unit 130. This driving and evaluationelectronic unit 130 can comprise for example one or a plurality ofelectronic components, for example a data processing unit, one or aplurality of volatile and/or nonvolatile memories and other components.

FIGS. 2A and 2B illustrate a schematic illustration of possibleexemplary embodiments of a sensor plaster 116 according to the inventionin different viewing directions. The sensor plaster 116 has a front side131, which, in a state in which the sensor plaster 116 has been appliedto a body surface (not illustrated in the figures), faces the bodysurface, and a rear side 133 facing away from the body surface. In thiscase, FIG. 2A shows a plan view of the front side 131 of the sensorplaster 116, whereas FIG. 2B shows a perspective view of the sensorplaster 116 highly schematically. In this perspective view, however, alayer construction is indicated symbolically, in a departure from theperspective illustration. The front side 131 is at the bottom in theillustration in accordance with FIG. 2B.

As emerges from the plan view of the front side 131 of the sensorplaster 116 in accordance with FIG. 2A, the sensor plaster 116 comprisesa flexible carrier element 134. Said flexible carrier element 134 can beconfigured in light-tight fashion, for example, and can serve as acarrier for the actual sensor module 136. By way of example, theflexible carrier element 134 can be configured in the form of arectangular, elongate strip and can comprise for example a carriermaterial comprising at least one flexible material and/or a layerconstruction of such flexible materials. By way of example, it ispossible here to use plastic materials, ceramic materials, papermaterials, glass materials or combinations of the aforementioned and/orother materials.

The carrier element 134 is intended to be configured flexibly in such away that it can be deformed in such a way that an adaptation to therespective body surface on which the measurement is intended to takeplace is possible. In this respect, the term “flexible” should beinterpreted as “deformable” in the context of the present invention.

As indicated by the dashed line in FIG. 2B, the carrier element 134 cancompletely cover the sensor module 136 on the rear side 133. However,just partial covering is also possible, in principle, for example if thesensor module 136 additionally comprises (see below) a solar cell,having a solar cell area facing toward the rear side 133.

The sensor module 136 has at least one active area 132 facing the frontside 131 and thus, in the applied state of the sensor plaster 116, thebody surface. Said active area 132 can also be configured in the form ofa plurality of individual areas. The active area 132 can comprise forexample one or a plurality of light-emitting areas of at least one lightsource 142, one or a plurality of detector areas of at least onedetector 146, one or a plurality of filters 144, 148, optical elements,protective elements or other components of the sensor module 136 and/orcombinations of the aforementioned elements and/or other elements of thesensor module 136.

The carrier element 134 has an adhesive surface 138, which completelyencloses the active area 132 in the exemplary embodiment in accordancewith FIG. 2A. The adhesive surface 138 can be configured as aself-adhesive adhesive surface 138 by means of an adhesive, for example.In particular, said adhesive surface 138 can in turn be configured insuch a way that, when the sensor plaster 116 has been stuck in place, noambient light can pass to the sensor module 136.

In the exemplary embodiment illustrated, the sensor module 136 has anoptical unit 140 as a bottommost—as viewed from the front side131—element of a layer construction. In the exemplary embodimentillustrated, said optical unit 140, the layer construction of which canbe discerned in FIG. 2B, for example, comprises a light source 142,which is configured as an organic light-emitting diode (OLED), forexample. An excitation filter 144, for example a filter film, can beapplied on said light source 142, such that said excitation filter 144faces toward the body surface.

In the exemplary embodiment illustrated, the optical unit 140furthermore comprises a detector 146, for example an organic solar cell.Said detector 146 is provided, on its side facing the active area 132,for example, with a response filter 148, for example once again in theform of a filter film adhesively bonded onto the detector 146.

As can be discerned from FIG. 2A and FIG. 2B, both the light source 142and the detector 146 are configured as large-area components, such thata large area of said components in each case faces the active area 132bearing directly on the body surface of the patient. By way of example,both the light source 142 and the detector 146 can have active areasfacing the body surface which comprise a few 10 mm², for example.However, smaller or larger areas are also possible, in principle. Inthis way, it is ensured that interrogation light is radiated onto thebody surface in a large-area manner and response light from the bodysurface can also be received in a large-area manner. Organic componentsare particularly well suited to such large-area components since, forexample in contrast to conventional inorganic semiconductor components,organic components by their nature are configured in large-area fashion.

In the next layer plane, on that side of the optical unit 140 whichfaces away from the active area 132, the sensor plaster 116 in theexemplary embodiment illustrated comprises an electronic unit 150. As analternative or in addition to the example illustrated in FIG. 2B,however, said electronic unit 150 can also be arranged in a differentway, for example wholly or partly alongside the optical unit 140.However, the layer construction illustrated can be realized particularlysimply in terms of printing technology, for example, and brings aboutshort electronic transmission paths and also a flat and compact design.The electronic unit 150 can comprise for example a driving electronicunit 152 for the driving and/or evaluation of the optical unit 140. Byway of example, by means of this driving electronic unit 152, the lightsource 142 can be excited to emit interrogation light and/or thedetector 146 can be excited to detect response light. Furthermore, thedriving electronic unit 152 can also comprise one or a plurality of datastorage devices in order to perform at least buffer-storage of themeasurement results that were obtained by means of the detector 146.Various other configurations are possible.

Furthermore, the sensor plaster 116 in accordance with the exemplaryembodiment illustrated in FIGS. 2A and 2B comprises a communication unit154, which can be configured for example wholly or partly as aninterface 156 for communication with the reader 118. Said communicationunit 154 can be configured using RFID technology, for example, and/orcan comprise a radiofrequency coil in order to realize the wirelesscommunication with the reader 118 as designated symbolically byreference numeral 128 in FIG. 1. The communication unit 154, too, can bedriven wholly or partly by the driving electronic unit 152 and/or canhave a separate driving electronic unit 152.

Furthermore, the sensor plaster 116 in the exemplary embodimentillustrated in FIG. 2B comprises an electrical energy source 158. Whilethe communication unit 154, the electronic unit 150 and the optical unit140 are arranged one above another in a layer design in the exemplaryembodiment illustrated in FIGS. 2A and 2B, which, however, likewise neednot necessarily be the case, the electrical energy source 158 isarranged alongside this layer construction in FIG. 2B. Alternatively oradditionally, however, the at least one electrical energy source 158 canalso be integrated fully or partly into the layer construction of theunits 140, 150 and 154.

The electrical energy source 158 can comprise for example a printedbattery, for example a printed polymer battery. The electrical energysource 158 can supply one or a plurality of the units 140, 150 and 154with electrical energy. As explained above, however, as an alternativeor in addition to the at least one electrical energy source 158, thesensor plaster 116 can also comprise one or a plurality of energygenerating devices, which are designated symbolically by the referencenumeral 159 in FIG. 2B. Said energy generating devices 159 can, asindicated symbolically in FIG. 2B, be configured jointly with theelectrical energy source 158, but can also be embodied wholly or partlyspatially separately from said electrical energy source 158.

By way of example, the required electrical energy can be radiated inexternally, in the manner used in conventional transponder technology.For this purpose, by way of example, the communication unit 154 canreceive its energy required for communication with the reader 118 fromthe incident electromagnetic waves. Alternatively or additionally, theenergy generating device 159 can also comprise for example one or aplurality of solar cells, for example once again one or a plurality oforganic solar cells. This at least one solar cell can then comprise forexample at least one solar cell area which faces the rear side 133 ofthe sensor plaster 116 and which is preferably at least not completelycovered by the carrier element 134, such that incidence of ambientlight, more particularly sunlight, onto said solar cell area ispossible. Once again alternatively or additionally, the energygenerating device 159 can comprise one or a plurality of thermoelectricconverters, for example one or a plurality of Peltier or Seebeckelements. Other configurations are also possible, or else combinationsof the aforementioned and/or other possibilities for the configurationof the energy generating device 159.

FIGS. 3 and 4 show possible exemplary embodiments of the light source142 (in FIG. 3) and of the detector 146 (in FIG. 4) in schematicperspective illustration. It should be pointed out that these layerconstructions are merely examples of a multiplicity of possible layerconstructions, and that materials other than those illustrated, otherlayer sequences, other layer thicknesses, other geometries or othertypes of production of the layers can also be used.

The light source 142 firstly comprises a substrate material 160. In theexemplary embodiment illustrated in FIG. 3, said substrate material 160is configured as transparent substrate material through which theinterrogation light 162 generated by the light source 142 can leave thelight source 142. In this respect, in the case of the layer constructionin accordance with FIG. 2B, said substrate material 160 has to face theactive area 132. It should be pointed out that, in order, for example,to be able to print the layer sequence of the light source 142 directlyonto the remaining layers of the layer construction illustrated in FIG.2B and/or to be able to print it directly onto the carrier element 134,the substrate material 160 can also be dispensed with or that saidsubstrate material 160 can be replaced by a different type oftransparent material. Such constructions are often also referred to asinverse constructions since, in the case of such constructions, thelayer sequence of the light source 142 is not actually applied to thesubstrate material 160 in the order illustrated, but rather in theopposite order. The designation “upside-down” layer construction is alsofound in this regard.

A transparent anode 164 is applied on the transparent substrate material160, which can comprise for example a glass, for example a thin,flexible glass, or optionally a transparent plastic material or acombination of these and/or other materials. Optionally, a differentelectrode than the anode can also be configured as a transparentelectrode. By way of example, indium tin oxide (ITO), for example havinga layer thickness of 30 to 80 nm, for example 50 nm, can be used astransparent anode material.

A barrier layer 166 can be applied to said transparent anode 164, whichbarrier layer can also be configured as a whole injection layer. By wayof example, this can be an oxide layer, having a thickness in the rangeof a few nanometers, for example 10 nm. For a possible construction ofsuch a whole injection layer, reference may be made to theabove-described publication by A. Pais et al.

A thin layer of a hole transport material 168 is applied to the barrierlayer 166. Said hole transport material 168, which has particularly highmobilities for positive charge carriers, for example radical cations,can be for example a layer of a few nanometers, for example 10 to 50 nm,of an N,N′-diphenyl-N,N′-bis(1-naphthyl)(1,1′-biphenyl)-4,4′diamine(NPB). Other hole transport materials or combinations of a plurality oflayers of different hole transport materials can also be used.

In the exemplary embodiment illustrated in FIG. 3, a layer of an emittermaterial 170 is applied on the hole transport material 168. In saidemitter material 170, the photons of the interrogation light 162 aregenerated by positive and negative charge carriers recombining thereand/or exciton pairs reacting and emitting photons in the process. Byway of example, said emitter material 170 can comprise a layer of a fewnanometers, for example 10 to 50 nm, of atris(8-hydroxyquinoline)aluminum (Alq). Other types of emitter materialsor combinations of different emitter materials can also be used.

In the exemplary embodiment illustrated, a layer of an electroninjection material is applied to the emitter material 170, said electroninjection material promoting electron injection into the emittermaterial 170 or of an electron transport material (not illustrated inFIG. 3) applied to the emitter material 170. By way of example, saidelectron injection material 172 can comprise a thin layer of a fluoride,for example lithium fluoride, for example with a layer thickness of 0.5to 2 nm, more particularly 1 nm. A cathode 174 is then applied to saidelectron injection material 172, from which cathode electrons areinjected into the organic layer construction. By way of example, it ispossible to use an aluminum cathode 174 having a layer thickness of 50to 200 nm, for example 100 nm. Other electrode materials can also beused in principle. If an inverse layer construction is used, in whichthe interrogation light 162 has to be emitted through the cathode 174,for example on account of the printing problem explained above, then thecathode 174, as an alternative or in addition to the anode 164, can alsobe configured in transparent fashion. This can be done for example byusing thin metal layers, for example in combination with transparentelectrode materials such as, for example, once again ITO.

Furthermore, it is indicated in FIG. 3 that the electrodes 164, 174 canbe suitably structured, if appropriate, in order to enable contact to bemade with said electrodes 164, 174.

The exemplary embodiment illustrated in FIG. 3 is an exemplaryembodiment of a light source 142 in which the active layers are producedcompletely from low molecular weight organic materials. Such lowmolecular weight organic materials are usually deposited from the gasphase. However, liquid phase deposition is also possible, in principle.It should be pointed out that other materials can also be used, and/orother deposition techniques, for example polymer materials, which can beapplied for example by a wet-chemical process. In the latter case, inparticular, a printing process or a method in which a plurality ofprinting processes are used is advantageous.

FIG. 4 shows, likewise only by way of example, an exemplary embodimentof a detector 146 in an illustration analogous to FIG. 3. It should onceagain be pointed out that other materials, other layer combinations, inparticular inverse constructions, constructions comprising additionallayers or other types of modifications of the layer construction shownare also possible.

The detector 146 in FIG. 4 is constructed as an organic photodiode. Thestarting point in the exemplary embodiment illustrated is once again asubstrate material 160, which can once again be configured intransparent fashion, for example, such that response light, which isdesignated by the reference numeral 176 in FIG. 4, can pass through saidsubstrate material 160 into the detector 146. It should once again bepointed out that, in the context of the present invention, inverseconstructions can also be used, that is to say constructions in whichthe response light 176 can pass into the detector 146 through atransparent top electrode (that is to say from above in FIG. 4) withoutpenetrating through the substrate material 160. Such a constructionwould be preferred for example in the context of a printing method foruse in a sensor plaster in accordance with FIG. 2B, in which, forexample, the layer sequence shown in FIG. 4 would be printed in aninverse order onto the light-opaque carrier element 134 illustrated inFIG. 2B. The light entrance of the response light 176 could then beeffected either via a transparent cathode or via a transparent anode,which would be arranged on that side of the layer construction whichfaces away from the carrier element 134 and faces the active area 132.In this respect, the statements made in respect of the organic lightsource 142 in accordance with FIG. 3 are analogously applicable to thedetector 146.

In the case of the exemplary layer construction in accordance with FIG.4, a transparent anode 164 is applied to the transparent substratematerial 160, which anode can once again comprise structured ITO forexample, which can be applied for example on a thin glass substrate 160or a thin plastic substrate 160.

A hole transport layer is applied to the ITO of the anode 164, said holetransport layer comprising for example a layer having a thickness of afew 10 nm, for example a layer having a thickness of 50 nm,poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS). Thislayer fulfills for example functions similar to those of the holetransport material 168 in accordance with FIG. 3, such that thereference numeral 168 has likewise been used for this hole transportlayer in FIG. 4.

In the exemplary embodiment illustrated in accordance with FIG. 4, adouble layer system of an acceptor-donor system comprising copperphthalocyanine 178 and the buckminsterfullerene C₆₀ 180 is applied tosaid hole transport layer 168. A mixed system in which said layers 178,180 are intermixed, for example, is also conceivable. While thefunctional principle of the organic light-emitting diode in accordancewith FIG. 3 is based on generation of photons upon recombination ofelectron-hole pairs (or the organic equivalents thereof), the functionalprinciple of the organic photodiode in accordance with FIG. 4 is basedon the opposite effect, in which photons entering into the componentgenerate electron-hole pairs (or the organic equivalents thereof).Finally, the C₆₀ layer 180 has applied to it an optional LiF layer 172and a structured cathode 174, for example an aluminum cathode, in asimilar manner to the construction in accordance with FIG. 3.

For further details of the possible exemplary embodiments which can beused in the context of the present invention, reference may be made tothe above-described publication by A. Pais et al.

It should furthermore be pointed out that the spectral properties of thecomponents in accordance with FIGS. 3 and 4 can be adapted to therespective requirements of the sensor plaster 116 in a simple manner.Thus, by way of example, the interrogation light 162 of the light source142 can be adapted to the respective requirements of the indicatorsubstance 112 or of a marker contained in said indicator substance 112.The illustrated component comprising Alq as emitter material emits inthe green spectral range, for example. However, it is possible toproduce components, for example by doping of the emitter material withsuitable dyes and/or by using other emitter materials which emit inother spectral ranges. By way of example, numerous organiclight-emitting diodes exist which emit in the short-wave visiblespectral range, that is to say for example in the blue spectral rangethrough to the near and ultraviolet spectral range. In this way, theinterrogation light 162 can be adapted for example to the respectiveabsorption characteristics of the indicator substance 112 or of a markerof said indicator substance 112. By way of example, emitter materialsexist which emit in the blue spectral range. By way of example, variousfluorine compounds as polymer materials emit in the blue spectral range.In the case of the low molecular weight emitter materials, Spirocompounds, for example, should be mentioned as possible emitters in theblue spectral range. Various other configurations and combinations ofdifferent emitter materials are possible.

Analogously, the spectral properties of the detector 146 can also beadapted to the response light 176 to be detected, such that optimumsignal generation can be effected. This can be done for example by usinga donor-acceptor system that differs from the donor-acceptor systemillustrated in FIG. 4. Various configurations are possible. It is alsopossible, for example, to use a plurality of light sources 142 havingdifferent spectral properties and/or a plurality of detectors 146 havingdifferent absorption characteristics, such that a simultaneousmeasurement in a plurality of spectral ranges can also be effected.

Finally, FIG. 5 shows an exemplary embodiment of a possible methodaccording to the invention for the transcutaneous measurement of anorgan function as a highly schematic flow chart.

The method begins in step 182 with the application of a sensor plaster116 to a body surface of a human or animal patient. This can be done,for example, by the adhesive surface 138, which can be configured as aself-adhesive adhesive surface, being stuck onto the body surface.

The method step 182 is followed optionally by a step of a zero valuemeasurement, which is designated by the reference numeral 183 in FIG. 5.This method step 183 serves the purpose of determining signals of thesensor plaster 116 before the indicator substance 112 is introduced.This can serve the purpose, for example, of eliminating electronicoffsets, background signals or the like, and/or defining a position ofthe coordinate axes. The results of the zero value measurement 183 canalso be used for other purposes. The zero value measurement 183 can beeffected, for example, by the step 186 (described below) of a detectionbeing carried out without the indicator substance 112 having beenintroduced into the body. It is also possible for this method step 186to be carried out a number of times. Furthermore, it is also possible tocarry out additional method steps, for example step 188 (likewisedescribed below) of storing information, for example storing the resultsof the zero value measurement 183.

Subsequently, in the example of the method according to the invention asillustrated in FIG. 5, there follows a method step 184, in which theindicator substance 112 is introduced into the patient's body. Thisintroduction can be effected, as explained above, for example by beingtaken orally, by injection or the like. It should be pointed out thatthis method step 182 need not necessarily be part of the method since,for example, it is also possible to use endogenous indicator substances112 which are present anyway in the body and the supply of which can beinterrupted, for example, or the regeneration of which can be blocked.Various configurations are conceivable.

Method step 186 involves detection of a concentration of the indicatorsubstance 112 in a body tissue and/or a body fluid of the patient bymeans of a transcutaneous measurement. By way of example, a measurementin interstitial fluid can be involved.

For the purpose of this detection 186, by means of the light source 142,interrogation light 162 is radiated through the body surface into thebody tissue or the body fluid, where a corresponding interaction withthe indicator substance 112 or a marker of said indicator substance 112is brought about, such that the response light 176 arises. Said responselight 176 is picked up by means of the detector 146. This gives rise toa first measurement signal, for example in the form of a measurementvalue pair, which can comprise, for example, the point in time of themeasurement or detection 186, the measured value of the response light176 (for example an intensity and/or a variable that correlates withsaid intensity, for example a photovoltage). Further data can also becontained in said measurement value pair, for example a luminance of thelight source 142 or a variable that correlates with said luminance, forexample a current through the light source 142.

These measurement results are stored in step 188. This storage can beeffected for example in an internal storage device of the sensor plaster116 or can, alternatively, or additionally, also be effected in astorage device of the reader 118. By way of example, the sensor plaster116, in particular the electronic unit 150 and/or the communication unit154, can comprise a volatile or nonvolatile memory, for example a flashmemory.

Subsequently, method steps 186 and 188 can be repeated, as indicated bythe reference number 190 in FIG. 5. The evaluation 192, which will beexplained in greater detail below, can also wholly or partly be aconstituent part of the repetition 190, this being indicated by thedashed line in FIG. 5. The repetition 190 can also be effected in such away that a predefined time is allowed to elapse between the individualrepetitions and/or that the repetitions take place at predefined pointsin time. In this way, by means of an N-fold repetition, a measurementseries can be recorded in which the detection 186 takes placecontinuously or discontinuously over a certain time period, for exampleat fixed or variable time intervals.

Subsequently, an evaluation is optionally effected in method step 192.This evaluation 192 can be effected in different ways and to differentdegrees. By way of example, the evaluation can already be wholly orpartly performed in the sensor plaster 116, for example in theelectronic unit 150, more particularly the driving electronic unit 152.Alternatively or additionally, however, an evaluation can also beeffected in the reader 118, there more particularly in the driving andevaluation electronic unit 130, and/or in a separate computer system,which can be connected to the reader 118, for example. A repetition isalso possible.

The evaluation can consist, for example, in a smoothing of themeasurement results, a filtering of the measurement results, anadaptation of measurement curves (for example in order to determine ahalf-life), a graphical representation or the like. A combination of theabovementioned steps and/or other evaluation steps is also conceivable.By way of example, the half-life and/or a renal clearance of theindicator substance 112 can be determined as the result of theevaluation 192. Other parameters are also conceivable.

EXAMPLE 2: PROPERTIES OF DEFINED INULIN MIXTURES

Defined inulin mixtures comprising 3 to 8 (F5) or 11 to 15 (F10) sugarmonomers were obtained from the raw material inulin by digestion with aninulinase and subsequent chromatographic separation into individualfractions.

The chromatographically separated fractions F5 and F10 were derivatizedwith fluorescein isothiocyanate (FITC) to form FITC-F5 and FITC-F10.FITC-F10 was administered to rats intravenously. The interstitialfluorescence of the FITC measured at an excitation wavelength of 485-520nm was determined. The fluorescence in the serum was measured as acontrol.

It was noticeable that with a reduction of the sugar residues the renalexcretion rate gradually decreased, with half-lives of 25.98+/−2.66 minfor FITC-F10 and 30.3+/−2.2 min for FITC-F5 compared with a half-life of25.02+/−1.67 min for sinistrin and 23.04+/−1.02 min for FITC-sinistrinand 22.0+/−0.8 min for the unmarked inulin F5 fraction.

The increase in the half-life can at least partly be explained by anincrease in the lipophilic properties of the molecules afterfluorescence marking. The marking efficiency for the F10 and F5fractions was, moreover, such that the fluorescence could even still bedetermined after drastic dose reduction by a factor of 10 or more. Theresults are illustrated graphically in FIGS. 6A to 6D and 7A to 7D.

FIGS. 6A to 6D illustrate recordings of a rat ear 194 which wereobtained using a small-animal imager of the CRI-Maestro type. Therecording times are 0 min (FIG. 6A), 1 min (FIG. 6B), 10 min (FIG. 6C),and 120 min (FIG. 6D). The fluorescent areas, discernible as bright inthe figures, correspond to the interstitial space 196 in the tissue.Regions without fluorescence mark the course of blood vessels 198.FITC-marked polyfructosans can therefore be measured transcutaneously inthe interstitial space, in principle.

FIGS. 7A to 7D show clearance experiments with FITC-markedpolyfructosans which were measured enzymatically or fluorometrically inplasma samples. In all the figures, the relative concentration c inpercent is plotted against the time t in minutes. FIG. 7A shows thedecrease in the relative concentrations of marked (FITC-S, measurementvalues represented as rhombi) and non-marked sinistrin (S, measurementvalues represented as squares) over time. FITC-S was administered torats as a bolus of 250 mg/kg body weight, and S as a bolus of 750 mg/kgbody weight. The half-lives for FITC-S and S are 23.9+/−1.4 min and22.8+/−1.4 min, respectively.

FIGS. 7B and 7C illustrate the decrease in the relative concentrationfor FITC-F10 (FIG. 7B) and FITC-F5 (FIG. 7C) over time and compared withthat of S (measurement values for FTTC-F10 and FITC-F5 are representedas rhombi, and those for S as squares). S was administered to rats as abolus of 750 mg/kg body weight, FITC-F10 as a bolus of 12 mg/kg bodyweight, FITC-F5 as a bolus of 14 mg/kg body weight. The half-lives inFIG. 7B are 24.5+/−1.4 min for FITC-F10 and 19.9+/−0.9 min for S, and inFIG. 7C 30.0+/−0.6 min for FITC-F5 and 21.0+/−0.1 min for S.

FIG. 7D shows a comparison of the decrease in the relativeconcentrations for marked (FITC-F5, measurement values represented asrhombi) and non-marked F5 inulins (F5, measurement values represented assquares) over time. FITC-F5 was administered as a bolus of 14 mg/kg bodyweight, and F5 as a bolus of 750 mg/kg body weight. The half-lives are29.5+/−1.5 min for FITC-F5 and 21.9+/−0.6 min for F5. A significantlybetter marking efficiency can be inferred from the lower bolusadministrations for FITC-F10 and FITC-F5 in comparison with FITC-S. Theincreased half-lives for FITC-F10 and FITC-F5 can be explained by thestronger lipophilic influence of the FITC group on the lipophilicproperties of the overall molecule.

List of reference symbols 110 Kit for the transcutaneous measurement ofan organ function 112 Indicator substance 114 Sensor system for thetranscutaneous measurement of an organ function 116 Sensor plaster forthe transcutaneous measurement of an organ function 118 Reader 120Operating elements 122 Indicator element 124 Interface 126 Radiofrequency interface 128 Wireless communication 130 Driving andevaluation electronic unit 131 Front side 132 Active area 133 Rear side134 Carrier element 136 Sensor module 138 Adhesive surface 140 Opticalunit 142 Light source 144 Excitation filter 146 Detector 148 Responsefilter 150 Electronic unit 152 Driving electronic unit 154 Communicationunit 156 Interface 158 Electrical energy source 159 Energy generatingdevice 160 Substrate material 162 Interrogation light 164 Transparentanode 166 Barrier layer 168 Hole transport material 170 Emitter material172 Electro injection material 174 Cathode 176 Response light 178 Copperphthalocyanin 180 C₆₀ 182 Application of sensor plaster 183 Zero valuemeasurement 184 Introduction of indicator substance 186 Detection 188Storage 190 Repetition 192 Evaluation 194 Rat ear 196 Interstitial space198 Blood vessels

1.-35. (canceled)
 36. A method for transcutaneously measuring the kidneyfunction in a subject, comprising using a kit (110), which comprises: atleast one sensor plaster (116), at least one sensor system (114), or acombination thereof; and wherein the sensor plaster comprises: (a) atleast one flexible carrier element (134) having at least one adhesivesurface (138) which can be stuck onto a body surface, (b) at least oneradiation source, wherein the radiation source is designed to irradiatethe body surface with at least one interrogation light (162), andwherein the radiation source is an integral constituent of the plaster,and (c) at least one detector (146), wherein the detector (146) isdesigned to detect at least one response light (176) incident from thedirection of the body surface; and (ii) at least one indicator substance(112) to be introduced into the blood of the subject such that atemporal concentration profile of the indicator substance in aninterstitial fluid in a tissue represents the kidney function.
 37. Themethod of claim 36, wherein the sensor plaster is designed to carry outa plurality of measurements at different points in time, and todetermine, from the measurement results of the plurality ofmeasurements, a temporal concentration profile and/or parameters derivedtherefrom, of an indicator substance in an interstitial fluid in atissue of the subject.
 38. The method of claim 36, wherein the indicatorsubstance comprises at least one marker, which emits at least oneresponse light upon incidence by at least one interrogation light fromthe radiation source of the sensor plaster.